pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0 carpet carpet - pGLO LB/amp 0 none none + pGLO LB/amp 130 pale yellow/grey grey + pGLO LB/amp/ara 35 light yellow neon green glow

2.	What two new traits do your transformed bacteria have?
	First of all the transformed bacteria grew in colony size by a lot because we only used one colony to begin with. While some grew, other bacteria without the pGLO plasmid were killed and some colonies glowed because of the addition of Ampicillin with the pGLO.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	There were 35 bacteria on the +pGLO LB/amp/ara plate because 35 colonies were created, same with 130 bacteria on the +pGLO LB/amp plate because each colony grows from a bacteria.
4.	What is the role of arabinose in the plates?
	Arabinose causes the bacteria to glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Detect Cancer: In animals, every cell that contains actin will glow green but the cells with cancer will glow red Transcription Reporter: placing GFP into a promoter will help track that organism’s gene expression Cell marker: GFP is used to see which cells have taken up a plasmid
6.	Give an example of another application of genetic engineering.

Another example of genetic engineering is plants that can fight pollution. Trees developed by scientists and the University of Washington can absorb polluted water through their roots and clean it before water is used and released in other forms.